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Site institutionnel d'Agrocampus OuestAGROCAMPUS OUEST

Institut supérieur des sciences agronomiques, agroalimentaires, horticoles et du paysage

Florence Baron

Florence BaronDépartement agroalimentaire
AGROCAMPUS OUEST
65, rue de Saint-Brieuc
CS 84215
F-35042 Rennes Cedex France


tel : +33 (0)2 23 48 56 94
fax : +33 (0)2 23 48 55 78
florence.baron@agrocampus-ouest.fr

Teaching
Research Topics
Main Publications
Links
French

After a PhD thesis in the ENSA Rennes (1999) on « the behaviour of Salmonella Enteritidis in egg white », I became assistant professor in food microbiology in the Laboratory of Microbiology in the Department of Food in AGROCAMPUS OUEST (UMR1253 INRA-AGROCAMPUS OUEST Science and Technology of Milk and Eggs). My general research area relates the microbiology of egg product. 

Teaching

- Course and practice on molecular biology and microbiological methods.
- Course, practice and case study in factories on food quality and safety. 

Research Topics

My research is focusing on the microbiology of egg products and on the relationship between micro-organisms and this food matrice. This research thematic was initiated by myself during my thesis and became extensive with the arrival of Sophie Jan in 2004. Our research is organized into two major areas.

The first area focuses on the study of the antimicrobial activity of egg white. Egg white is recognized as a protecting environment for embryo with a lot of bacteriostatic and bactericidal activity not yet understood. We want to know what molecular structures or complex molecular systems interact with micro-organisms and are involved in the anti-microbial activity of egg white and what are the mechanisms and the physiological targets involved in bacteria.

The second area concerns the control and hygienic improvement of egg products. We study the influence of technological processes and hen breading practices on the contamination of egg products to propose relevant ways of control. Currently, our research focuses on bacteria belonging to the Bacillus cereus group. Some strains of this group produce toxins in foods (emetic) and / or in the human intestine, which may induce emesis or diarrhoea, respectively. They also present a broad spectrum of enzymatic activities that may be responsible for egg product spoilage, even at low temperature when psychrotrophic strains are involved.
We try to better understand the level of eggshell contamination, the species encountered, their ability to germinate, to grow and produce toxin at low temperatures in the egg products, and their ability to colonize the industrial surfaces.
The aim is to get new insights on the risk associated with these bacteria in the sector of egg production and to consider relevant ways to control contamination. The control of this risk is a major competitive challenge for egg product manufacturers.

Main Publications

- Journal articles :

Baron F, Nau F. Guérin-Dubiard C., Gonnet F., Dubois J.J., Gautier M. 2003. Effect of dry heating on the microbiological quality, functional properties and natural bacteriostatic ability of reconstituted egg white. J. Food Prot. 66 (5) : 825-832.

Le Loir Y., Baron F., Gautier M.. 2003. Staphylococcus aureus and food poisoning.. Genet. Mol. Res. 2 (1): 63-76.

Jeantet R., Carballeira Fernández J.A., Roignant M., Cochet M.F., Baron F., Korolczuk J.,Brion J.C. 2003. Performances d’un nouvel appareil de décontamination microbienne de produits liquides en continu. Industries Alimentaires et Agricoles. Avril 2003.

Vidal M.-L., Baron F., Ahmed A., Michel J., Gautron J., Beaumont C., Gautier M., Nys Y. 2003. Genetic variability of anti-microbial activity of hen’s egg white. British Poultry. Science 44 (5):791-2.

Baron F., Briandet R., Lesne J., Humbert F., Ablain W., Gautier, M. 2004. Influence of a non favourable environment : egg white on resistance to heat and disinfectant, bioadhesive properties and virulence of Salmonella Enteritidis. J. Food Prot. J Food Prot. 67(10):2269-73

Jeantet J., Ripoll Mc Keag J., Carballeira Fernández J., Grosset N., Baron F., Korolczuk J. 2004. Continuous pulsed field applied to egg products . Science des aliments 24(2) 137-158

Baron F., Cochet M.-F., Pellerin J.-L., Ben Zakour N., Lebon A., Navarro A., Proudy I., Le Loir Y., Gautier M. 2004. Development of a PCR test for the differentiation between Staphylococcus aureus and Staphylococcus intermedius. J. Food Prot. 67 (10) : 2302-5.

Korolczuk J Ripoll Mc Keag J., Carballeira Fernández J., Baron F., Grosset N., R.,Jeantet J. 2006. Effect of pulsed electric field processing parameters on Salmonella enteritidis inactivation. Journal of food engineering. 75 : 11-20.

Baron F., Cochet M. F., Ablain W., Grosset N., Madec M. N., Gonnet F., Jan S., Gautier M. 2006. Rapid and cost-effective method for microorganism enumeration based on miniaturization of the conventional plate-counting technique. Le Lait 86 251-257.

Sellier N., Vidal M.L., Baron F., Michel J., Gautron J., Protais M., Beaumont C. Gautier M., Nys Y. Estimations of repeatability and heritability of different measures of egg’s natural defenses. British Poultry Science 48(5) : 559-66.

Baron F., Cochet M.F., Grosset N., Madec M.N., Briandet R., Dessaigne S., Chevalier S., Gautier M., Jan S. 2007. Isolement and characterization of a psychrotolerant and toxin producer Bacillus weihenstephanensis in liquid egg products. Journal of Food Protection 70 (12) : 2782-2791.

- Thesis :

Baron F., 1994. Développement et utilisation d'une sonde oligonucléotidique ciblant l'ARN ribosomal 16S pour l'étude des populations d'archaea dans l'écosystème digestif de l'homme. Mémoire de DEA de l'Ecole Nationale Supérieure Agronomique de Rennes et de l'Université des Sciences de Rennes.

Baron F., 1998. Etude du comportement de Salmonella Enteritidis dans le blanc d'œuf. Thèse de Doctorat de l'Ecole Nationale Supérieure Agronomique de Rennes.

- Book chapters

Baron F., Réhault S., Nau F. Compounds with antibacterial activity , In: Huopalathi R., Lopez-Fandino R., Anton M., Schade R. (Eds.), Bioactive egg compounds: characterization and application, Springer, Heidelberg, 2007.

Baron F. and Gautier M., Evaluation des caractéristiques physico-chimiques et de la qualité des aliments : évaluation microbiologique, In: Jeantet R., Croguennec T., Schuck P., Brulé G. (Eds.), Science des aliments, Vol. 1 : Stabilisation biologique et physico-chimique, Tec & Doc Lavoisier, Paris, 2006, pp. 352-364.

Baron F. and Gautier M., Altérations microbiennes, In: Jeantet R., Croguennec T., Schuck P., Brulé G. (Eds.), Science des aliments, Vol. 1 : Stabilisation biologique et physico-chimique, Tec & Doc Lavoisier, Paris, 2006, pp. 59-94.

Baron F. and Gautier M., Maîtrise de la qualité des aliments, In: Jeantet R., Croguennec T., Schuck P., Brulé G. (Eds.), Science des aliments, Vol. 1 : Stabilisation biologique et physico-chimique, Tec & Doc Lavoisier, Paris, 2006, pp. 343-351.

- Oral Communications :

Baron F., Cochet M.-F., Pellerin J.-L., Benzakour N., Lebon A., Navarro A., Proudy I., Le Loir Y., Gautier M. Développement d’un test PCR pour la différentiation entre Staphylococcus aureus et Staphylococcus intermedius.Congrès de la Société Microbiologique de France, Paris, novembre 2003.

Baron F. et R. Jeantet. Egg product preservation processes : a review. X European Symposium on the Quality of Eggs and Egg Products. septembre 2003, Ploufragan, France. Baron F.: Antimicrobial properties of egg white. Workshop of COST Action 923 17-19 Octobre 2004 Barcelone, Espagne (Conférence invitée)

Baron F.* et Jan S. Microbiologie de l’œuf et des ovoproduits. Congrès de la branche Française de la WPSA, 23 mars 2006, Le Mans, France (conférence invitée). Baron F. * et Jan S. (Conférence invitée) : Egg and egg product microbiology : actual consideration on breeding and technological practicesWorkshop of COST Action 923 28-30 Juin 2006 Turku, Finlande

Jan S.*, Baron F., Gautier M. Growth and cytotoxic activity of Bacillus weihenstephanensis in liquid egg products at low temperatures. B. cereus meeting, 21–22 mai 2007, Guyancourt, France (conférence invitée).

Jan S.*, Cochet M.F., Grosset N., Madec M.N., Briandet R., Dessaigne S., Chevalier S., Gautier M., Baron F. Isolation of a spore-forming, psychrotolerant Bacillus weihenstephanensis in liquid egg products stored at low temperatures : study of growth, cytotoxicity, and ability to stick and form biofilms. II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007), 28 nov-1 déc 2007, Séville, Espagne.

* lecturer

Links

UMR INRA-AGROCAMPUS OUEST Sciences et Technologie des Aliments (STLO)